PT and APTT

PT (Prothrombin Time) 

1) Pre warm the PT reagent 10 minutes at 37°C.

2) Take 100 ul of plasma into the test tube wait for 30 seconds. 

3) Add 200 ul of reagent in the test tube. Put it the water bath and start the stop watch. Stop it when the clot is formed take out the tube and look for the visible clot. 

*Calculate the INR on the system. 

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APTT (Activated Partial Thromboplastin Time) 

1) Separate test tube contain in Cephascreen and Calcium Chloride solution should be brought to 37°C (keep in water bath 5 - 10 minutes) 

2) To test tube add 100 ul plasma and 100 ul of Cephascreen shake tube briefly. To mix the reagent and plasma and place tube at 37°C for 3 - 5 minutes. 

3) Add Forcibly 100 ul pre warmed Calcium chloride into plasma and simultaneously start the stopwatch. Shake tube keep at 37°C for 20 seconds. Incubation remove the tube. Gently tilt back and forth until a gel clot form stop the watch and record time.